Increase in green, renewable and sustainable energy demand due to higher environmental impacts (e.g. Greenhouse gases emissions, climate change, etc.) on consumption of fossil fuel resource put down an extra pressure on government, researchers and industrialists. Among several available biofuel options, biohydrogen is considered as one of the best environmentally clean fuel and a strong candidate to fulfil the future demand of sustainable energy resource. Although, biohydrogen production technology and its use as a fuel is still in infancy stage. Selection of most sustainable production pathway, increase in production upto industrial scale and cost efficiency are some issue still persist with the biohydrogen research.